MBGP TILLING consortium
Aims
- To facilitate screening of large populations in several Brassica species by:
- reciprocal exchange of DNA pools
- an agreed protocol for access to seed
- notification of when populations will be "grown out" for phenotype assessment
- To provide a public database of relevant populations and of identified mutations in "TILL"ed genes
- To exchange experience between member labs and publish protocols on website
Current MBPG TILLING initiatives
| Species |
Genotype |
Method |
M1 lines |
M2 lines |
Calc. mutant load |
Contact |
Institution |
Country |
| napus |
Tanto |
0.5% EMS |
3,000 |
|
|
Nathalie Nesi |
INRA |
France |
| |
Tanto |
0.5% EMS |
6,200 |
|
|
Nathalie Nesi |
INRA |
France |
| |
Ningyou 7 |
0.3% EMS |
1,000 |
2-3 from each |
|
Jinling Meng |
HAU |
China |
| |
Ningyou 7 |
0.6% EMS |
3,800 |
2-3 from each |
|
Jinling Meng |
HAU |
China |
| |
DH12075 |
0.25-0.4%EMS |
3,000 |
3,000 |
|
George Haughn |
UBC |
Canada |
| |
Zhongshuan 9 |
0.2% EMS |
98 |
|
|
Shengyi Liu |
IOCR |
China |
| |
Zhongshuan 9 |
0.5% EMS |
6,400 |
|
|
Shengyi Liu |
IOCR |
China |
| |
Zhongshuan 9 |
0.8% EMS |
139 |
|
|
Shengyi Liu |
IOCR |
China |
| rapa |
Chiifu |
0.6% EMS |
714 |
3,000 |
|
Yong Pyo Lim |
CNU |
Korea |
| |
R-O-18 |
0.25% EMS |
1,600 |
|
|
Lars Ostergaard |
JIC |
U.K. |
| |
R-O-18 |
0.3% EMS |
3,600 |
7,000 |
|
Lars Ostergaard |
JIC |
U.K. |
| |
R-O-18 |
0.4% EMS |
1,600 |
3,000 |
|
Lars Ostergaard |
JIC |
U.K. |
| |
R-O-18 |
Fast neutron 60 Gy |
270 |
3,500-5,000 |
|
Lars Ostergaard |
JIC |
U.K. |
| |
R-O-18 |
5AzaCytidine |
|
|
|
Graham King |
RRes |
U.K. |
| |
rapid cycling |
EMS optimisation |
|
|
|
Scott Woody |
U. Wisconsin |
USA |
| oleracea |
? |
? |
|
|
|
Chris Pires |
U. Missouri |
USA |
| |
var. capitata |
? |
|
|
|
Zhuang Mu |
IVF |
China |
Publications
Web links
Diversity Fixed Foundation Sets
Related resources
This Page Was Last Edited: 25-Mar-2010